Veterinary Assistant II
Company: Tree House Humane Society
Location: Chicago
Posted on: February 14, 2026
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Job Description:
Job Description Job Description Veterinary Assistant Effective
1/1/2026 Position Summary: The Veterinary Assistant supports Tree
House’s Veterinary Services department by providing high-quality,
compassionate care to shelter animals and community pets.
Veterinary Assistants ensure animals move safely and efficiently
through medical, surgical, and clinic workflows while maintaining a
clean, organized, and low-stress environment. Depending on skill
level (I–III), this role includes hands-on animal handling,
medication and treatment administration, diagnostic and surgical
support, and participation in population-health and
disease-prevention efforts. Veterinary Assistants collaborate
closely with veterinarians, certified veterinary technicians,
behavior staff, animal care teams, and other departments to uphold
excellent medical care, efficient operations, and a culture of
teamwork. Staff in all levels are expected to use Fear Free
handling practices, follow strict biosecurity standards, and
contribute to a positive, learning-focused environment that
supports both animals and people. Core Responsibilities (All
Levels): Safely and humanely handle animals using Fear Free–aligned
restraint techniques, accommodating a wide range of temperaments
and health conditions. Assist with daily rounds, treatment
administration, and population monitoring. Maintain sanitation of
all medical areas following disease control protocols. Restock and
organize treatment, iso, and surgical areas Support veterinarians,
management, and certified technicians during exams, treatments,
spay/neuter surgeries, and other procedures Accurately document
treatments and observations in medical records Support intake
processing and discharge of animals, ensuring proper identification
and care instructions Follow infection control, zoonotic disease
prevention, and biosecurity protocols Contribute to maintaining a
calm, safe, and compassionate environment for both animals and
staff Foundational: Veterinary Assistant I Overview: Entry-level
role focusing on foundational skills in animal handling,
sanitation, and supporting daily clinic operations. Core
Competencies: Safely handle and restrain healthy, injured, fearful,
or fractious animals under supervision, following Fear Free–aligned
techniques Perform daily cleaning, disinfection, and laundry
according to clinic and organizational protocols Assist with
setting up and breaking down surgical procedures. Assist with
preparing treatment and intake stations Accurately read and record
animal identification information and historical medical records;
(kennel cards, microchip numbers, treatment sheets) Assist with
intake exams, including holding animals, preparing vaccines,
labeling samples, and organizing necessary supplies Administer
basic medications under direct supervision (oral, topical, and
approved subcutaneous treatments) Perform subcutaneous injections
(vaccines, fluids) with guidance from a veterinarian or certified
technician Support basic venipuncture tasks, including restraint,
site preparation, and performing simple blood draws under
supervision when trained Capture diagnostic radiographs following
safety, positioning, and radiation protection protocols Prepare
animals and equipment for radiographic procedures, including PPE,
positioning aids, and logging Participate reliably in high-volume
medical workflows such as vaccine clinics, intake processing, and
daily treatments Assist with surgical preparation, clipping,
aseptic skin prep, and assisting with patient positioning Assist
with sample collection and processing (fecal samples, urine
collection using non-invasive methods, blood tubes, cytology slide
preparation) Clean and prepare surgical instruments and packs for
sterilization Monitor animals during and after procedures at a
basic level—recording TPRs, observing recovery, and promptly
reporting concerns Maintain accurate logs and documentation
(radiology logs, cleaning logs, instrument tracking, controlled
drug logs as assigned) Restock medical areas and maintain clean,
organized workspaces Use personal protective equipment correctly
and consistently Provide basic animal husbandry support (feeding,
watering, litter maintenance) as needed to support medical flow
Safely transport animals between kennels, treatment areas, surgery,
and recovery following clinic protocols Apply basic first-aid
interventions under supervision (wound cleaning, simple bandage
changes, flea/tick treatments) Participate in continuing education,
skill development, and clinic training opportunities Recognize and
promptly report abnormal animal behaviors or medical concerns to
supervising staff Contribute to a calm, safe, and compassionate
environment while demonstrating growth toward independent
low-stress handling Training & Experience: High school diploma or
equivalent required. Previous animal handling experience is
preferred but not required. Completion of clinic onboarding and
Level 1 skills checklist. Intermediate: Veterinary Assistant II
Overview: Demonstrates increased technical skills, confidence with
clinic medical workflows, and the ability to support population
health with minimal direct supervision. Veterinary Assistant II
staff perform more complex procedures, provide reliable patient
monitoring, and help maintain efficient daily operations. Core
Competencies: Administer oral, topical, intramuscular, and
subcutaneous medications and vaccines independently as directed
Perform more advanced sample collection tasks, including fecal,
skin, hematology, and urine collection (free catch, litterbox,
expression when trained) and preparing samples for diagnostic
testing Assist with venipuncture beyond basic support (restraining,
holding off, collecting blood from common sites when trained)
Capture high-quality radiographs with correct positioning and
safety practices, including basic troubleshooting (retakes,
adjusting exposure factors under guidance) Assist with surgical
preparation, including IV site prep, clipping, aseptic skin prep,
and assisting with patient positioning Monitor patients recovering
from anesthesia or sedation, recording TPRs, assessing pain, and
alerting veterinary staff to abnormalities Perform more advanced
patient observations, including monitoring for dehydration, pain,
GI upset, respiratory distress, and behavioral decline Accurately
enter and update medical information in clinic software systems
(treatments, notes, flow-through tasks, medical flags) Recognize
signs of common shelter diseases (URI, parvo, panleukopenia,
ringworm, calicivirus), understand basic transmission risks, and
implement appropriate isolation or biosecurity steps Perform
intermediate husbandry or supportive care tasks, including
administering SQ fluids independently, assisting with basic bandage
changes, setting up IV fluid therapy, or completing routine wound
care under direction Communicate effectively with veterinarians,
certified technicians, and shelter operations staff to support
population flow and medical priorities Maintain organized,
well-stocked medical areas and identify needed inventory
replenishment or equipment maintenance Mentor and support Level 1
assistants, volunteers, and new staff, modeling appropriate
handling and safety protocols Participate in continuing education,
skill development, and clinic training opportunities Training &
Experience: 2–3 years of experience in a shelter or veterinary
setting. Demonstrated mastery of Level 1 skills and ongoing
application of Fear Free handling Successful completion of Level 2
in-house training and competency evaluations Professional
Expectations (All Levels): Maintain a compassionate, calm, and
professional demeanor when interacting with animals, clients,
volunteers, and colleagues Prioritize safety, biosecurity, and
population health by consistently following infection-control, PPE,
animal-handling, and sanitation protocols Promote and model Fear
Free and low-stress handling practices in all interactions with
animals Work collaboratively across departments, demonstrating
awareness of daily workflow, organizational capacity, and
internal/public population needs Communicate clearly, respectfully,
and proactively with teammates and leadership to support efficient
operations and continuity of care Participate in continuing
education, professional development, and required training,
demonstrating a commitment to ongoing skill growth Support a
culture of learning, respect, and teamwork, including constructive
problem-solving, openness to feedback, and helping others succeed
Maintain reliable attendance, punctuality, and adherence to clinic
policies, contributing to consistent and predictable care for
animals Uphold confidentiality, ethical conduct, and professional
standards in all aspects of work Demonstrate flexibility and
adaptability in a dynamic, high-volume environment, adjusting to
changing priorities and animal care needs Advancement Pathway:
Level Focus Supervision Typical Timeframe* Title 1 Foundational
medical skills High supervision 0–12 months Veterinary Assistant I
2 Technical skills & workflow mastery Moderate supervision 24-36
months Veterinary Assistant II 3 Advanced & leadership
responsibilities Low supervision 36 months Veterinary Assistant III
*Timeframes are general guidelines; advancement depends on
demonstrated skills, behaviors, readiness, and organizational
needs. Optional Add-Ons (Specialty Skill Training Tracks): Surgery
Track High-volume spay/neuter support, including efficient patient
flow, prep, and recovery Advanced anesthesia assistance and
monitoring for advanced surgical and dental procedures Surgical
instrument care, pack assembly, autoclave operation, and equipment
troubleshooting Post-operative recovery and monitoring, pain
scoring, and discharge support Infectious Disease Track Isolation
ward management, including daily rounds, treatment coordination,
and sanitation oversight Biosecurity leadership: PPE protocols,
barrier nursing, and staff training. Support for outbreak response,
surveillance testing, and cohorting strategies Monitoring
population health indicators and communicating risks to medical
leadership Behavior Track Safe handling and behavioral support for
challenging, fearful, fractious, or undersocialized animals
Implementation of behavior plans, enrichment protocols, and
stress-reduction strategies Assistance with behavior-related
medication administration and tracking. Collaboration with the
behavior department on monitoring, documentation, and
behavior-forward flow Foster & Intake Support Track Intake triage,
including health assessments, vaccine/parasite protocols, and
client-facing communication Foster coordination support, including
educational guidance, supply prep, and case follow-up Support for
foster-to-adopt and neonatal programs (as trained) Clear,
empathetic communication with clients, fosters, and partner
organizations to support population flow Scope of Practice
Clarification (Illinois): Veterinary Assistants are unlicensed
assistive personnel. All medical tasks are performed only under the
supervision and delegation of a licensed veterinarian, in
accordance with the Illinois Veterinary Medicine and Surgery
Practice Act. While Veterinary Assistants may carry out delegated
clinical support functions, Certified Veterinary Technicians hold
exclusive responsibility for advanced clinical procedures—including
dentistry, anesthesia monitoring, diagnostic interpretation, and
other functions defined as the practice of veterinary medicine
under Illinois law. The above is intended to describe the general
content of and requirements for the performance of this job. It is
not to be construed as an exhaustive statement of duties,
responsibilities or physical requirements. Nothing in this job
description restricts management’s right to assign or reassign
duties and responsibilities to this job at any time. Reasonable
accommodations may be made to enable individuals with disabilities
to perform the essential functions.
Keywords: Tree House Humane Society, Hammond , Veterinary Assistant II, Healthcare , Chicago, Indiana